Anti-ulcer Activity of Ethanolic Extract of Alstonia scholaris Bark: An in vivo and in silico Approach

Aims: The present research is focused on screening in vivo anti-ulcer activity using pylorus ligation and ethanol induced ulcer model. 
 Study Design: Fourty eight rats, randomly divided into groups, were used this study. Bark of Alstonia scholaris air-dried, ground fine powder the preparation an ethanolic extract.
 Place Duration Study: Department Pharmacology, Osmania University, Hyderabad between December 2020 September 2021.
 Methodology: Ethanol related was 1 mL/kg b.w, p.o. Treated rats received extract at various doses 200 400 mg/kg b.w. Ulcer index, % protection calculated histological studies conducted 6 hr after pylorous respectively.
 Results: Pharmacological estimations done by means mg/kg, total acidity free decreased, pH increased index decreased Ethanolic (EEAS b.w., p.o.) Treatment with EEAS (200 has significantly To appreciate ligand-binding attraction dynamic ingredients excerpt, docking trainings accomplished for natural compounds against protein data bank (PDB) ID: 5A5N, PDB 6Q2T, 7MBX, 2FV5. results revealed that vanillic acid, venoterpine, loganetin, dibutyl phthalate, guaia-3,9- diene, 3,6-Bis[2-methylphenyl]-2,5-dihydropyrrolo[3,4-c] pyrrole-1,4- dione, 2H-1-Benzopyran- 2-one,7-acetyl-8-[acetyloxy]-4, n-hexadecanoic stigmasterol, diospyrolide, D- Friedoolean-14-en-3-one, betulin, lupeol acetate, pentanoic acid standard drug omeprazole had shown highest glide scores all selected proteins which indicate a stronger receptor-ligand binding affinity.
 Conclusion: From silico it evident bark possessed significant activity.